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Communication Dans Un Congrès Année : 2023

Omics approaches highlight milk molecules impacting offspring early gut maturation

Résumé

OBJECTIVES: Milk composition is complex and includes numerous components essential for offspring growth and development. In addition to the high abundance of miR-30b microRNA, milk produced by the transgenic mouse model of miR-30b-mammary deregulation displays a significantly altered fatty acid profile. Moreover, wild-type adopted pups fed with this milk present an early growth defect. This study aimed to investigate the consequences of miR-30b milk feeding on neonatal gut development, a prime target of suckled milk, along with comprehensive milk phenotyping. METHODS: The duodenum of wild-type pups fed miR-30b milk was extensively characterized at postnatal day-5, day-6 and day-15 using histological, transcriptomic, proteomic, and intestinal permeability analyses, and compared to pups fed wild-type milk. Milk of miR-30b foster dams collected at mid-lactation was extensively analyzed using proteomic, metabolomic, lipidomic approaches and hormonal immunoassays. RESULTS: At postnatal day-5, pups fed miR-30b milk showed maturation of their gut tissue (increase expression of claudins and changes in eight duodenal protein), with an earlier reduction in paracellular and transcellular permeability. Compared to wild-type milk, miR-30b milk displayed an increase in total lipid, ceramide and sphingomyelin levels, the over-expression of nine proteins involved in the gut barrier, and higher insulin and leptin levels. CONCLUSIONS: Integrative systems biology with omics approaches highlight significant changes of bioactive components in miR-30b milk associated with neonatal gut integrity and maturation, that could be involved in the earlier intestinal closure phenotype of the pups, which is associated with a lower growth rate.
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Dates et versions

hal-04227272 , version 1 (03-10-2023)

Identifiants

  • HAL Id : hal-04227272 , version 1

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Sandrine Le Guillou, Céline Ciobotaru, Johann Laubier, Johan Castille, Etienne Aujean, et al.. Omics approaches highlight milk molecules impacting offspring early gut maturation. International Milk Genomics Consortium (IMGC) Symposium, International Milk Genomics Consortium, Davis, CA, USA, Sep 2023, Cork, Ireland. ⟨hal-04227272⟩
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