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Article Dans Une Revue Peptides Année : 2007

New Trichobrachins, 11-residue peptaibols from a marine strain of Trichoderma longibrachiatum

Gaëtane Wielgosz-Collin
Laurence Poirier
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Olivier Grovel
Karina Petit
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Mustapha Mohamed-Benkada
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Thibaut Du Pont
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John Bissett
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Philippe Vérité
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Gilles Barnathan
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Yves Pouchus
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Karina Ethel Petit
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Thibaut Robiou Du Pont
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Yves François Pouchus

Résumé

A marine strain of Trichoderma longibrachiatum isolated from blue mussels (Mytilus edulis) was investigated for short peptaibol production. Various 11-residue peptaibols, obtained as microheterogenous mixtures after a chromatographic fractionation, were identified by positive mass spectrometry fragmentation (ESI-IT-MSn, CID-MSn and GC/EI-MS). Thirty sequences were identified, which is the largest number of analogous sequences so far observed at once. Twenty-one sequences were new, and nine others corresponded to peptaibols already described. These peptaibols belonged to the same peptidic family based on the model Ac-Aib-xxx-xxx-xxx-Aib-Pro-xxx-xxx-Aib-Pro-xxol. They were named trichobrachin A when the residue in position 2 was an Asn, and trichobrachin C when it was a Gln. Major chromatographic sub-fractions, corresponding to purified peptaibols, were assayed for their cytotoxic activity. Trichobrachin A-IX and trichobrachin C exhibited the highest activities. There was an exponential relation between their relative hydrophobicity and their cytotoxicity on KB cells.

Dates et versions

hal-03595727 , version 1 (13-04-2022)

Identifiants

Citer

Nicolas Ruiz, Gaëtane Wielgosz-Collin, Laurence Poirier, Olivier Grovel, Karina Petit, et al.. New Trichobrachins, 11-residue peptaibols from a marine strain of Trichoderma longibrachiatum. Peptides, 2007, 28 (7), pp.1351-1358. ⟨10.1016/j.peptides.2007.05.012⟩. ⟨hal-03595727⟩
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