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              <p>The three-dimensional analysis of sample by Serial Block Face Imaging finds its fields of application in the study of micro-anatomy and developmental biology. The concept is to produce a large amount of images directly from the surface of a block in which the sample is included, as it is cut (microtome or cryostat). Serial images are already pre-aligned and allow reconstructing the sample in three dimensions and exploring it in any virtual cutting plane. The MicroPICell platform and the company Kaer Labs are collaborating to develop this technique, by combining a removable camera with an integrated LED excitation source, positioned in front of a conventional microtome or cryostat. From organs included in colored paraffin or frozen in OCT, this technique allowed us to acquire serial images thanks to the fluorescence contrast between the sample and the embedding medium. We could then make a simplified 3D reconstruction of these samples, keeping their structures. Compared to the commercial systems available, this system is inexpensive, requires simple sample preparation and is adaptable for both paraffin and frozen samples.</p>
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              <p>L’analyse tridimensionnelle d’un échantillon par Serial Block Face Imaging trouve ses champs d’application dans l’étude de la micro-anatomie et la biologie du développement. Le concept est de produire une grande quantité d’images directement à partir de la surface d’un bloc dans lequel est inclus l’échantillon, et ce, au fur et à mesure de sa coupe (au microtome ou cryostat). Les images sériées sont de fait déjà pré-alignées et permettent de reconstruire en trois dimensions l’échantillon et de l’explorer selon n’importe quel plan de coupe virtuel. La plateforme MicroPICell et la société Kaer Labs collaborent afin de reproduire cette technique, avec une caméra amovible comprenant une source d’excitation LED intégrée, positionnée en face d’un microtome ou d’un cryostat classique. A partir d’organes soit inclus en paraffine colorée soit congelés et enrobés dans de l’OCT, cette technique nous a permis d’acquérir des images en série grâce au contraste de fluorescence entre l’échantillon et le milieu d’enrobage. Nous avons pu ensuite faire une reconstruction 3D de ces échantillons, en conservant leurs structures. Par rapport aux systèmes commerciaux disponibles, ce système est peu onéreux, demande une préparation simple des échantillons et est adaptable aussi bien pour des échantillons inclus en paraffine qu’en congelé.</p>
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          <date type="start">2018</date>
        </org>
      </listOrg>
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</TEI>